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March 5, 2021
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March 5, 2021
Published
March 5, 2021
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Abstract

Class 1 integrons play a role in the emergence of resistant bacteria by facilitating the recruitment of gene cassettes encoding antibiotic resistance genes. inhibition zone diameters were determined in tests with imipenem (IPM) and meropenem (MEM) discs alone and in combination with 1000 µg of EDTA Combined disk diffusion method was used for detection of integron CS type metallo-β-lactamase-producing isolates, they were emplace each of imipenem and meropenem antibiotics with EDTA on Mueller-Hinton agar, because EDTA working to inhibit of metallo-β-lactamase enzymes and increase the diameter of inhibition zones. The results revealed that out of 0 ∕ 22 (0 %) isolates produce these enzymes with imipenem and out of 0 ∕ 22 (0 %) isolates produce these enzymes with meropenem. All the 22 isolates were submitted to molecular detection of Integron CS by using PCR assay. The results showed that only 1 (4.5%) isolates were carried Integron CS.                        

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References

  1. Berge`s, L. ; Rodriguez-Villalobos, H. ; Deplano, A. and Struelens, M. (2007) Prospective evaluation of imipenem/EDTA combined disc and E-test for detection of metallo-β-lactamase producing Pseudomonas aeruginosa . J Antimicrobial Chemother., 50 : 812-813.
  2. CLSI: Clinical and Laboratory Standards Institute (2007). Performance Standards for antimicrobial susceptibility testing, seventeenth informational supplement, CLSI document M100-S17, Wayna, PA. USA
  3. Collis, C.M. and Hall, R.M. (1992). Mobile gene cassettes and integrons : capture and spread of genes by site-specific recombination . Mol Microbiol., 6: 2875- 2885.
  4. Johnson, J.R. ; Kuskowski, M.A. ; Menard, M.; Gajewski, A. Xercavins, M. and Garau, J. (2006). Similarity between human and chicken Escherichia coli isolates in relation to ciprofloxacin resistance status. J. Infect. Dis. 194:71–78.
  5. MacFaddin, J. (2000) . Biochemical tests for identification of medical bacteria . Lippincott Williams & Wilkins. Philadelphia, USA.
  6. Mehedi, M. H. (2010). Detection of Integrons from Escherichia coli Isolates Obtained from Humans and Animals in the Republic of Korea. Thammasat Int. J. Sc. Tech., 15: 48-52.
  7. Pitout, D. ; Barbara, L. ; Daniel, B. ; Kevin, B. ; Sameer E. and Deirdre, L. (2007). Molecular Epidemiology of metallo-β-lactamase-producing Pseudomonas aeruginosa in the Calgary Health Region: Emergence of VIM-2-Producing Isolates. J Clin Microbiol., 43: 294-298.
  8. Ploy, M. ; Chainier, D. ; Tran Thi, N. ; Poilane, I. ; Cruaud, P. ; Denis, F. ; Collignon, A. and Lambert, T. (2003). Integron-associated antibiotic resistance in Salmonella enterica serovar Typhi from Asia. Antimicrob. Agents Chemother., 47:1427–1429.
  9. Recchia, G. D. and Hall, R. M. (1995). Gene cassettes: a new class of mobile element. Microbial., 141: 3015-3027.
  10. Stokes, H. and Hall, R. (1989) . A novel family of potentially mobile DNA elements encoding site-specific gene integration functions: integrons. Mol Microbiol., 3: 1669–1683.
  11. Toussaint, A. and Merlin, C. (2002). Mobile Elements as a Combination of Functional Modules. Plasmid 47: 26–35.
  12. Yong, D. ; Lee, K. ; Yum, J. ; Shin, H. ; Rossolini, G. and Chong, Y. (2002). Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of pseudomonas spp. and Acinetobacter spp. J Clin Microbiol., 40: 3798-3801.
  13. Zhang, R. ; Eggleston, K. ; Rotimi, V.and Zeckhauser, R. J. (2006). Antibiotic resistance as a global threat: evidence from China, Kuwait and the United States. Global Health 7:2–6

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