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February 28, 2020
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Abstract

A case control study was conducted in Thi-Qar governorate, south of Iraq, included a total 154 individuals 104 of theme were infertile patients while the other 50 males were fertile healthy control males, who attended the infertility center in AAl- Hussein Teaching hospital, from 20/10/ 2016 to 16/1/ 2018.  aged between (l8-5l) years with mean of age was (29.95±0.68) and (50) healthy controls males aged between (23-37) years with mean age was (27.58±0.51). All patient submitted full reports about their medical status history, in addition to their infertility type (primary or secondary). Seminal fluid samples were collected by masturbation after three days of sexual avoidance. All patients and healthy control males were tested for presence of anti-spermatozoa antibodies in their seminal fluid. seminal fluid analysis in this study showed there were significant differences (p < 0.05) between fertile control group and infertile males (patients) in (The seminal fluid viscosity, Liquefaction time, Semen volume, Sperm motility and total sperm account  while the results revealed there were no significant differences in the seminal fluid parameters between infertile patients and fertile control group in semen color, pH and sperm morphology. The overall prevalence of ASA in seminal plasma among studied papulation was 16.23% (25/l54). Among healthy control group anti-sperm antibody in seminal plasma were positive in 6% (3 /50) and negative in 94% (47/50) with mean concentration of anti-sperm antibody was 24.97±3.7 U/ml (normal range 0 – 60 U/ml). Among infertile patients anti-sperm antibody was positive in 21.15% (22/104) whereas negative in 78.84 % (82/104). The mean concentration of anti-sperm antibody was 40.71±2.32 U/ml. The statistical analysis revealed highly significant differences (P <0.05) in presence of ASA in seminal plasma of infertile patients compered to normal fertile males, also concentrations of ASA in seminal plasma was significantly higher in patients compered to healthy control males. In conclusion ASA  was proportionally high among patient with infertility  compered to healthy fertile control group.

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